EFEITO DO DIFENOCONAZOL SOBRE POPULAÇÕES DE Trichoderma spp.: UM ESTUDO COMPARATIVO
DOI :
https://doi.org/10.33837/msj.v9i1.1769Mots-clés :
EC50, sensibilidade, bioinsumo, isoladosRésumé
Fungos do gênero Trichoderma spp. são amplamente distribuídos, conhecidos por atuar como agentes de biocontrole, absorção de nutrientes, promoção de crescimento vegetal, decomposição da matéria orgânica, resistência ao estresse hídrico e salino e resistência sistêmica induzida (ISR) nas plantas. O objetivo deste trabalho foi determinar a dosagem letal e sensibilidade de isolados de Trichoderma spp. oriundos de diferentes hospedeiros ao difenoconazol. Utilizou-se 16 isolados de Trichoderma spp., que foram coletados de campos produtores de soja localizadas no Estado de Goiás, Paraná e Brasília. Estes isolados estão preservados no laboratório de Fitopatologia do IFGoiano - Campus Urutaí. Utilizou-se meio de batata-dextrose-ágar (BDA) para obtenção de culturas monospóricas. Foram utilizando concentrações de (0, 0.1, 1, 10 e 100 µL mL-1), com um delineamento experimental inteiramente casualizado (DIC), com três repetições por tratamento, totalizando 240 unidades experimentais. Avaliou-se o diâmetro da colônia do 1 ao 7 dia após a inoculação (DAI), avaliou-se a área abaixo da curva de progresso do crescimento micelial (AACPCM), taxa de crescimento micelial (TCM), eficácia de controle a 50% de crescimento micelial (EC50) e a % de inibição do crescimento. A EC50 variou entre os isolados de 0,26 ± 1,18 (IF 299) a 4,40 ± 7,63 µL mL-1(IF 310) (EC50 ± erro padrão). Com base nessa amplitude, pode-se dizer que os isolados são sensíveis ao difenoconazol, comparando com outras populações que alcançam EC50 em patamares de 80-200 µL mL-1 (ppm). Por outro lado, uma proporção minoritária dos isolados (6,25%) mostrou-se insensível. O isolado IF 316 apresentou menor AACPCM e consequente maior sensibilidade as diluições de difecononazol.
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© Leidy Ximena Figueroa Cossio, Ana Luiza da Silva Mendes, Yamile Salvatierra Nuñez, Ana Carolina Pereira Fonseca, Ricardo Rodrigues Junior, Luiz Fillipe Martins Melo, Carlos Gabriel Xavier Dourado, Luiza Pereira Costa, Mileny da Silva Luiz, Ana Claudia dos Santos, Walter Baida Garcia Coutinho, Rejane Araújo Guimarães, Erica Fernandes Leão Araújo, Melina Korres Raimundi, Milton Luiz da Paz Lima 2026
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