Effect of difenoconazole on populations of Trichoderma spp.: a comparative study
DOI:
https://doi.org/10.33837/msj.v9i1.1769Keywords:
EC 50, sensitivity, bioinput, isolatesAbstract
Fungi of the genus Trichoderma spp. are widely distributed and recognized for their role as biological control agents, as well as for contributing to nutrient uptake, plant growth promotion, organic matter decomposition, tolerance to water and salt stress, and induction of systemic resistance (ISR) in plants. The objective of this study was to determine the lethal dose and sensitivity of Trichoderma spp. isolates from different hosts to the fungicide difenoconazole. Sixteen isolates collected from soybean-producing fields in the states of Goiás, Paraná, and the Federal District, Brazil, were evaluated. These isolates are preserved in the Plant Phytopathology Laboratory of the IF Goiano – Urutaí Campus. Monosporic cultures were obtained on potato-dextrose-agar (PDA). The isolates were exposed to difenoconazole concentrations of 0, 0.1, 1, 10, and 100 µL mL-1 in a completely randomized design with three replications, totaling 240 experimental units. Colony diameter (mm) was measured from the 1st to the 7th day after inoculation, and the area under the mycelial growth progress curve (AUMGPC), mycelial growth rate (MGR), effective concentration to inhibit 50 % of mycelial growth (EC50), and percentage of growth inhibition were calculated. EC50 values ranged from 0.26 µL mL-1 (IF 299) to 4.40 µL mL-1 (IF 310), indicating high sensitivity of most isolates to difenoconazole when compared with populations that show EC50 values between 80 and 200 µL mL-1. Only 6.25 % of the isolates showed insensitivity to the fungicide. The isolate IF 316 presented the lowest AUMGPC, indicating greater sensitivity to difenoconazole concentrations.
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Copyright (c) 2026 Leidy Ximena Figueroa Cossio, Ana Luiza da Silva Mendes, Yamile Salvatierra Nuñez, Ana Carolina Pereira Fonseca, Ricardo Rodrigues Junior, Luiz Fillipe Martins Melo, Carlos Gabriel Xavier Dourado, Luiza Pereira Costa, Mileny da Silva Luiz, Ana Claudia dos Santos, Walter Baida Garcia Coutinho, Rejane Araújo Guimarães, Erica Fernandes Leão Araújo, Melina Korres Raimundi, Milton Luiz da Paz Lima
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