Identificação fenotípica e genotípica de cepas de estafilococos oriundas de uma unidade de abate de aves
DOI :
https://doi.org/10.33837/msj.v1i2.85Résumé
Vários tipos de alimentos são implicados em intoxicações gastrointestinais por Staphylococcus aureus, sendo frequentemente relacionado com carne e derivados, devido às propriedades de atividade de água, pH, nutrientes e a extensiva manipulação destes alimentos durante o processamento. Este trabalho teve por objetivo identificar cepas potencialmente toxigênicas de Staphylococcus isoladas de diferentes nichos em uma unidade de abate de frango de corte, bem como avaliar a resistência à diferentes antimicrobianos. De um total de 120 isolados, 75 foram considerados como típicos do gênero Staphylococcus, sendo 63,7% coagulase positiva e 37,3% coagulase negativa. Pela técnica de Reação em Cadeia de Polimerase (PCR) confirmou-se que 86,67% dos isolados eram pertencentes ao gênero Staphylococcus pela detecção do gene tuf e 77,33% da espécie S. aureus pela amplificação do gene nuc. Verificou-se a presença do gene sec em duas cepas, sendo uma delas estafilococos coagulase negativa. A maioria dos isolados foram resistentes à sulfonamida (98,5%), ácido nalidíxico (89,3%) e penicilina G (87,70%). Dentre os isolados resistentes à penicilina, 4,60% foram oxacilina resistentes e 1,5% vancomicina resistentes.
Palavras-chave: Staphylococcus aureus, coagulase negativa, resistência à antibioticos, enterotoxinas
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