Seasonal variations in the seminal plasma composition of male goats

Auteurs

  • Julio Cesar Oliveira Dias Universidade Federal de Viçosa http://orcid.org/0000-0002-9356-4264
  • Cristina Mattos Veloso
  • Madriano Christilis da Rocha Santos Santos
  • Carlos Thiago Santos Alvim Mendes de Oliveira
  • Camila Oliveira Silveira
  • Erick Iglesias
  • Pedro Henrique Furtado
  • Rita Flávia Miranda de Oliveira Donzele

DOI :

https://doi.org/10.33837/msj.v1i7.339

Mots-clés :

eletroforese, minerais, proteínas, espermatozoide

Résumé

Este estudo avaliou as variações mensais proteicas e bioquímicas no plasma seminal de bodes da raça Alpina criados em clima tropical. A avaliação da motilidade progressiva de espermatozóides foi realizada no sêmen fresco e descongelado, e a avaliação da funcionalidade da membrana após a descongelação. Após 12 meses, o plasma seminal foi submetido a eletroforese em gel de SDS-poliacrilamida a 10 e 14%, em paralelo com as análises dos seus parâmetros bioquímicos. No gel de poliacrilamida a 10% foram identificadas 22 bandas proteicas  (25-181 kDa), enquanto que no gel de 14%, foram identificadas 16 bandas proteicas (5,7-165 kDa). As frações de proteínas de 5,7 e 34,3-34,5 kDa mostraram um perfil que variou de acordo com a sazonalidade reprodutiva dos bodes, com o aumento da produção ocorrendo durante a estação reprodutiva. Na análise dos parâmetros bioquímicos, as concentrações de cálcio, fósforo, e colesterol também mostraram variação sazonal. No entanto, a análise da motilidade progressiva, bem como a funcionalidade de membrana não alteraram. Estes resultados indicam que alterações nos perfis proteico e bioquímico do plasma seminal durante o ano, não alterou a qualidade do sêmen fresco e descongelado de bodes Alpinos em clima tropical.

Biographie de l'auteur

Julio Cesar Oliveira Dias, Universidade Federal de Viçosa

Médico Veterinário atuando na área de Reprodução Animal (Fisiologia e Biotecnologias), com ênfase em ruminantes (bovinos, caprinos e ovinos)

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Publiée

2018-03-18

Comment citer

Dias, J. C. O., Veloso, C. M., Santos, M. C. da R. S., de Oliveira, C. T. S. A. M., Silveira, C. O., Iglesias, E., Furtado, P. H., & Donzele, R. F. M. de O. (2018). Seasonal variations in the seminal plasma composition of male goats. Multi-Science Journal, 1(7), 34–40. https://doi.org/10.33837/msj.v1i7.339

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Technical Communications

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